A synaptic mechanogenetic technology to repair brain connectivity

Introduction

We aim to develop a mechanogenetic technology to regulate functional connectivity of neural circuits and show how it can be harnessed for therapeutic purposes in high-prevalence treatment-resistant brain disorders.

Background

Mechanogenetics is an emerging field of health science that attempts to regulate neural networks by combining the advantages of optogenetics with those of magneto-mechanical stimulations.

• Like optogenetics, it relies on targeted actuators to achieve circuit specificity.
• It exploits magnetic fields to remotely stimulate the brain.

Yet, despite solid theoretical foundations and encouraging experimental results, we are to date unable to repair a dysfunctional brain using mechanogenetics due to technological barriers in spatial resolution and in vivo implementation.

Proposed Solution

We propose an innovative solution based on:

1. Functionalized biocompatible magnetic nanoparticles
2. Bioengineered synaptic mechanosensors

These components synergistically integrate at specific synaptic connections to bidirectionally regulate brain circuit connectivity in response to focused magnetic fields of different frequencies delivered via high-permeability transcranial magnetic stimulators.

By hijacking the signaling pathways of synaptic mechanosensors, we aim to promote a normalization of neural circuit activity that outlasts the therapeutic intervention. We will assess the capacity of the synaptic mechanogenetic toolkit to promote and depress network activity in mouse models of stroke and epilepsy.

Interdisciplinary Consortium

To achieve this ambitious goal, we have gathered an interdisciplinary consortium comprising:

• Material scientists
• Electronic experts
• Physiologists
• Clinicians

Our approach, based on magnetic fields that penetrate brain tissue unimpeded, is predicted to go beyond current therapeutic paradigms because it does not require the implantation of invasive devices. At the same time, it promises to achieve subcellular resolution for repairing connectivity defects that underlie most brain disorders.