PRInted Symbiotic Materials as a dynamic platform for Living Tissues production

Introduction

The PRISM-LT aims at creating a flexible platform for next generation living tissue manufacturing based on Hybrid Living Materials.

Bio-Ink Design

We plan to design a novel bio-ink where stem cells are integrated in a support matrix enriched with engineered helper cells (either bacteria or yeasts, depending on the application and requirements).

Bioprinting Process

Tuning the operational parameters of the bioprinting process, we will cast down the material controlling the mechanical properties of each voxel. This will result in a 3D patterned structure where stem cells are locally induced to initiate their differentiation towards different lineages.

Stem Cell and Helper Cell Interaction

As far as stem cells proliferate, the helpers remain in a quiescent state. However, when the stem cells get stimulated by the local (printed) mechanics and enter a differentiation pathway, they start secreting a pool of lineage-specific metabolites.

The helper cells are designed to:

1. Sense these early markers of differentiation.
2. Respond by producing in-situ the corresponding growth factors, providing the relevant chemical guidance.

Helper cells within the platform amplify the initial lineage commitment in each area and dynamically sustain differentiation on a longer term.

Project Implementation

During the project, we will implement this strategy and develop two independent symbiotic materials, targeting biomedical and food applications, respectively.