SubsidieMeestersPaving the way for High-throughput Organoid ENgineering using Integrated acoustiX
PHOENIX aims to develop a microfluidic technology for high-throughput generation of vascularized cerebral organoids, enhancing reproducibility and maturation through acoustophoresis and two-photon writing.
Projectdetails
Introduction
The aim of PHOENIX is to use my expertise in microsystems engineering to close critical technology gaps in organoid generation. Cerebral organoids are 3D self-assembled structures derived from human induced pluripotent stem cells, replicating both structure and function of the human foetal brain. Organoids have the potential to replace existing 2D cell cultures and animal models, but this has not yet been realized due to rudimentary preparation methods.
Technology Gaps
In PHOENIX, three important technology gaps will be addressed:
- Reproducibility
- Controlled maturation
- Vascularisation
I aim to build on my pioneering research on droplet acoustofluidics and the scientific output of my ERC Starting Grant to develop three microfluidic modules that, at the end of the project, shall be integrated into a seamless organoid engineering pipeline.
Methodology
The technology in focus is acoustophoresis, a method to manipulate particles and cells by ultrasound. This will be used to achieve ordered encapsulation of stem cells in hydrogel droplets and develop a microfluidic platform where the cells can be differentiated under fully controlled conditions.
Finally, two-photon writing will be used to integrate a vascular network with the organoid constructs to form an important delivery architecture for nutrients and blood components. PHOENIX will be focused on both technology development and thorough biological characterization of the resulting organoids to demonstrate both expected and unexpected benefits of transferring organoid generation on-chip.
Collaborations
Collaborations have been established with:
- Prof. Christine Mummery and Dr. Valeria Orlova, both at LUMC, NL
- Dr. Anna Falk at KI, SE
These collaborations will provide expertise in complementary fields of this highly interdisciplinary project.
Expected Output
The expected output of PHOENIX is a microfluidic technology that enables high-throughput generation of cerebral organoids with a multi-regional structure and vascularisation in a direct process.
Financiële details & Tijdlijn
Financiële details
| Subsidiebedrag | € 2.832.500 |
| Totale projectbegroting | € 2.832.500 |
Tijdlijn
| Startdatum | 1-4-2023 |
| Einddatum | 31-3-2028 |
| Subsidiejaar | 2023 |
Partners & Locaties
Projectpartners
- UPPSALA UNIVERSITETpenvoerder
Land(en)
Vergelijkbare projecten binnen European Research Council
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|---|---|---|---|---|
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High Throughput Modelling and Measurement of Human Epithelial Models using Electrospun Conducting Polymers For Unlocking Data-Driven Drug DiscoveryThe project aims to enhance drug discovery by developing simplified Organ on Chip platforms through hydrogel electrospinning, enabling scalable monitoring and integration into industry workflows. | ERC Proof of... | € 150.000 | 2025 | Details |
Directed Orchestration of Microfluidic Environments for guided Self-organisationThe project develops the DOMES microfluidic platform to study environmental impacts on kidney organogenesis, enhancing understanding of congenital anomalies through advanced 3D cell culture models. | ERC Proof of... | € 150.000 | 2022 | Details |
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Reprogramming of somatic cells into organOids: patient-centred neurodevelopmental disease modelling from nascent induced pluripotency
The project aims to develop a robust method for generating human brain organoids from patients with Fragile X Syndrome to explore neurodevelopmental phenotypes and inform targeted therapies.
High Throughput Modelling and Measurement of Human Epithelial Models using Electrospun Conducting Polymers For Unlocking Data-Driven Drug Discovery
The project aims to enhance drug discovery by developing simplified Organ on Chip platforms through hydrogel electrospinning, enabling scalable monitoring and integration into industry workflows.
Directed Orchestration of Microfluidic Environments for guided Self-organisation
The project develops the DOMES microfluidic platform to study environmental impacts on kidney organogenesis, enhancing understanding of congenital anomalies through advanced 3D cell culture models.
Inducing functionality in retinal organoids with electrical activities derived from developing retina
This project aims to enhance the functionality of retinal organoids by using electrophysiological insights from mouse retina development and mathematical models to induce naturalistic electrical features.
An automated platform for the large-scale production of miniaturized neuromuscular organoids
The project aims to automate and scale the production of complex neuromuscular organoids for high-throughput drug screening to advance therapies for neuromuscular diseases.
Vergelijkbare projecten uit andere regelingen
| Project | Regeling | Bedrag | Jaar | Actie |
|---|---|---|---|---|
Origami Paper-based tecHnology fOr the innovativE aNd sustaInable Organ-on-Chip devicesThe PHOENIX-OoC project aims to revolutionize Organ-on-Chip technology by developing origami paper-based devices for cell co-cultures and pharmacological studies, enhancing sustainability and functionality. | EIC Pathfinder | € 2.202.333 | 2024 | Details |
Revolutionary high-resolution human 3D brain organoid platform integrating AI-based analyticsThe 3D-BrAIn project aims to develop a personalized bio-digital twin of the human brain using advanced organoid cultures and machine learning to enhance precision medicine for CNS disorders. | EIC Pathfinder | € 1.998.347 | 2023 | Details |
IDEFIX Multiorgan toxicity and efficacy test platformCherry Biotech's IDEFIX project aims to revolutionize preclinical drug testing by developing a customizable organ-on-chip platform that mimics human multiorgan physiology, enhancing efficacy and toxicity predictions. | EIC Transition | € 2.496.073 | 2022 | Details |
Next Generation 3D Tissue Models: Bio-Hybrid Hierarchical Organoid-Synthetic Tissues (Bio-HhOST) Comprised of Live and Artificial Cells.Bio-HhOST aims to create bio-hybrid materials with living and artificial cells for dynamic communication, enhancing tissue modeling and reducing animal use in drug research. | EIC Pathfinder | € 1.225.468 | 2024 | Details |
High-throughput ultrasound-based volumetric 3D printing for tissue engineeringSONOCRAFT aims to revolutionize myocardial cell construct bioprinting by combining rapid volumetric printing with ultrasonic manipulation to create functional cardiac models for drug testing and disease research. | EIC Pathfinder | € 2.999.625 | 2025 | Details |
Origami Paper-based tecHnology fOr the innovativE aNd sustaInable Organ-on-Chip devices
The PHOENIX-OoC project aims to revolutionize Organ-on-Chip technology by developing origami paper-based devices for cell co-cultures and pharmacological studies, enhancing sustainability and functionality.
Revolutionary high-resolution human 3D brain organoid platform integrating AI-based analytics
The 3D-BrAIn project aims to develop a personalized bio-digital twin of the human brain using advanced organoid cultures and machine learning to enhance precision medicine for CNS disorders.
IDEFIX Multiorgan toxicity and efficacy test platform
Cherry Biotech's IDEFIX project aims to revolutionize preclinical drug testing by developing a customizable organ-on-chip platform that mimics human multiorgan physiology, enhancing efficacy and toxicity predictions.
Next Generation 3D Tissue Models: Bio-Hybrid Hierarchical Organoid-Synthetic Tissues (Bio-HhOST) Comprised of Live and Artificial Cells.
Bio-HhOST aims to create bio-hybrid materials with living and artificial cells for dynamic communication, enhancing tissue modeling and reducing animal use in drug research.
High-throughput ultrasound-based volumetric 3D printing for tissue engineering
SONOCRAFT aims to revolutionize myocardial cell construct bioprinting by combining rapid volumetric printing with ultrasonic manipulation to create functional cardiac models for drug testing and disease research.