Decoding communication between mobile genetic elements

Introduction

Communication between organisms is one of the most sophisticated forms of social interactions. It has been recently discovered that bacterial viruses (phages) show multiple social attributes including the ability to communicate extracellularly with their progenies through a peptide-based system called arbitrium.

Arbitrium System

This system is utilized by phages to guide life-cycle decisions, such as the transitions between their quiescence and virulence states. It has subsequently become clear that this system is not exclusive to phages but is also present in other mobile genetic elements (MGEs) and that its underlying design and function have diversified considerably.

Research Gaps

Despite the wide-ranging impact of these discoveries, the molecular basis of these new systems of communication, and their function in different MGEs, remain to be deciphered. More importantly, although it has been proposed that phages only communicate with their progeny, our preliminary results indicate that the arbitrium system can be used by phages and other elements to communicate with unrelated MGEs, sometimes present in different bacterial species.

Ecological Role

However, the prevalence and ecological role of this unprecedented inter-MGE communication is unclear.

Project Overview

In the TalkingPhages project, we will explore all these questions and establish the molecular basis used by arbitrium systems to shape MGE diversity, interactions amongst MGEs, and bacterial ecology and evolution.

Team Expertise

Our team combines expertise in MGEs and communication systems using tools from:

1. Genetics (Penads)
2. Biochemistry (Marina)
3. Eco-evolutionary dynamics (Eldar)

Project Significance

By exposing the multi-layered network of communications between MGEs, which is amenable for exploration on multiple levels of resolution, this project would set a milestone in understanding the complex eco-evolutionary dynamics of MGEs and the relevance of their sociality. This has potential impact on our understanding of virulence and resistance in clinically and agriculturally important bacterial species.