SubsidieMeestersReading DNA in real time for medical applications
The project aims to develop a high-throughput, real-time DNA analysis method using Laser-Assisted DNA Optical Mapping for liquid biopsies and biomedical applications, enhancing service and automation.
Projectdetails
Introduction
The aim of this project is to use an ultra-fast, high throughput method for analyzing DNA single molecules for liquid biopsy and other biomedical applications.
Methodology
The proposed methodology, “Laser-Assisted DNA Optical Mapping (LADOM)”, allows retrieving the barcode of single molecules of DNA in real time, as they flow through a nanochannel in a fluidic device.
Key Features
This method combines several advantages:
- Cheap device fabrication
- Flexible DNA labeling (customizable for different applications)
- Microscope- and camera-free setup
- Read-out sensitive to single molecules
- Very high throughput (tens of molecules per minute)
- Ability to detect very small fragments
- No limitation for the maximum molecule’s length
These features are especially interesting for applications dealing with ultra-long intact genomic DNA.
Funding Request
In this project, we request funding to:
- Offer a service to analyze samples from possible stakeholders in the biomedical sector
- Standardize the technology
- Improve the throughput by developing software to automate the data analysis
Financiële details & Tijdlijn
Financiële details
| Subsidiebedrag | € 150.000 |
| Totale projectbegroting | € 150.000 |
Tijdlijn
| Startdatum | 1-10-2022 |
| Einddatum | 31-3-2024 |
| Subsidiejaar | 2022 |
Partners & Locaties
Projectpartners
- UNIVERSITY OF HAMBURGpenvoerder
Land(en)
Geen landeninformatie beschikbaar
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