BioCHIPS - Biofabricated microfluidcs CHIPS based on self assembling of CNCs to recreate the hierarchical fibrillar structure of human tissues ECM

Introduction

Biochips proposes an innovative bottom-up strategy to directly fabricate cell-laden devices that recreate the unique biophysical cues from the native fibrillar ECMs and allow the design of bioengineered microtissues with arbitrary geometries.

Platform Overview

The proposed platform combines the concepts of matrix-assisted 3D free-form bioprinting with the controlled self-assembly of colloidal cellulose nanocrystals (CNCs) to fabricate cell-laden constructs embedded within its own fibrillar CNC hydrogel device.

High-Throughput Capability

The proposed platform can:

1. Array multiple independent single organ models in a high-throughput manner (the number will depend on the desired model complexity and well plate used).
2. Link multiple tissue/organ models together with microfluidic circuits that can be user-defined on their CAD designs.

Features of the BioCHIPS System

The BioCHIPS system enables:

• High-resolution printing of complex and perfusable multicellular constructs without separating membranes or plastic barriers.
• Interaction between cells through signaling gradients created by compartmentalization in a bioinspired fibrillar matrix, supporting their long-term culture.

Additional Advantages

In addition to optical transparency for real-time monitoring, CNCs hydrogels can be bioorthogonally digested to release the embedded constructs for post-bioprinting analysis and processing. This is a crucial advantage in organ/tissue-on-chip applications.

Applications

Beyond the fabrication of perfusable microfluidic channels and cell-laden chambers for the development of 3D microphysiological systems as in vitro models, the intrinsic characteristics of this bioinspired platform further enable its scale-up to produce tissue-engineered constructs within its own bioreactor for in vitro maturation and biological tests at higher scales.