Advanced 3D in vitro models based on magnetically-driven docking of modular microscaffolds

Introduction

This project is focused on the design, the production, the characterization, and the proposal for future commercialization of 3D modular co-culture systems, specifically designed to recapitulate the physio-pathological microenvironment of brain tumors.

Key Technology

The key technology at the base of the proposed project is the design of magnetic microscaffolds and their fabrication through two-photon polymerization (2pp). This is a disruptive mesoscale manufacturing technique that enables low-cost obtainment of microstructures with nanometric resolution, characterized by unprecedented levels of accuracy and reproducibility.

Biohybrid Device

A microtubular structure scaffolding endothelial cells and connected to a fluidic system will be exploited to mimic the blood-brain barrier. This biohybrid device will serve as the base for the assembly of ferromagnetic microcages hosting glioblastoma cells. It will also be provided with docking systems for superparamagnetic microcages carrying undifferentiated and differentiated neuronal progenitor cells.

Innovation

This approach represents a disruptive innovation compared to other 3D models available in the literature. It will allow a faithful recapitulation of the complex glioblastoma microenvironment through a platform that can be very easily handled in any laboratory.

Future Applications

High-throughput screenings of brain drugs and in vitro testing of the efficacy of different anticancer therapies are envisaged upon successful accomplishment of the project. This will lead to a pioneering generation of flexible multi-cellular platforms easily adaptable to the mimicry of different pathological conditions.