A new framework to interrogate molecular mechanisms mediating antagonistic diatom-bacteria interactions

DIACIDAL aims to uncover the mechanisms of bacterial pathogenicity towards diatoms and their defense responses, enhancing understanding of oceanic carbon fluxes and potential biotechnological applications.

Subsidie
€ 2.299.893
2025

Projectdetails

Introduction

Interactions between phytoplankton and bacteria play key roles in mediating carbon and nutrient fluxes in the oceans, yet the mechanistic basis and environmental impacts of such interactions are poorly understood. DIACIDAL addresses crucial unknowns in our understanding of the interactions of one of the most globally abundant phytoplankton, the diatoms, and their bacterial pathogens.

Background

This proposal builds on my team's recent advances in developing an innovative sampling pipeline to isolate environmentally relevant bacterial pathogens of diatoms. This work suggests that bacterial pathogenicity towards diatoms is more prevalent than recognized previously.

New Evidence

Moreover, we have important new evidence that diatoms can sense bacterial pathogens to evade their attack. Our new framework will be coupled with cutting-edge molecular tools that I have pioneered to study diatom signaling, including biosensors to track real-time signaling responses in single diatom cells. These tools have already made important breakthroughs in our understanding of how diatoms sense their environment.

Methodology

Exploiting these advances, we will use culture-dependent and new single-cell culture-independent approaches, including:

  1. Tn-Seq
  2. Comparative omics
  3. Imaging

By focusing on a model interaction, we will decipher mechanisms mediating bacterial pathogenicity towards diatoms and elucidate how diatoms sense and evade their pathogens. This will reveal novel effectors and defense signaling systems.

Ecosystem Impact

Finally, we will leverage the new tools and insights to quantify the prevalence of bacterial pathogenicity and diatom defense in natural ecosystems. This innovative, multidisciplinary program will advance understanding of mechanisms governing bloom dynamics, inform ecosystem models, and shed light on a poorly characterized environmental stressor confronting phytoplankton communities.

Potential Applications

This also has the potential to unlock new translational opportunities for biotechnology, such as novel antimicrobial and anti-fouling compounds.

Financiële details & Tijdlijn

Financiële details

Subsidiebedrag€ 2.299.893
Totale projectbegroting€ 2.299.893

Tijdlijn

Startdatum1-8-2025
Einddatum31-7-2030
Subsidiejaar2025

Partners & Locaties

Projectpartners

  • THE UNIVERSITY OF EXETERpenvoerder
  • MARINE BIOLOGICAL ASSOCIATION OF THE UNITED KINGDOM

Land(en)

United Kingdom

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