Understanding the molecular basis of protein folding disorders and protein quality control in muscle

Introduction

Mutations in certain proteins can cause their misfolding and aggregation, posing a potential threat to the health of an organism. Protein aggregation is counteracted by protein quality control (PQC) mechanisms that reduce the amounts of damaged molecules.

Aging and Protein Misfolding Diseases

As we age, however, the crucial surveillance mechanisms weaken and become less effective, leading to protein misfolding diseases. The most well-known protein disorders, or proteinopathies – Alzheimer's, Huntington's, and Parkinson's diseases – are characterized by the formation of toxic protein aggregates, known as amyloids.

Hidden Protein Disorders

Current research focuses on such amyloid diseases; however, there seem to be many other hidden protein disorders. In principle, every mutation in a protein can affect its folding and stability and may become pathological only when PQC mechanisms are compromised.

Research Objectives

To better understand this kind of proteinopathy, we will generate novel PQC tools and study muscle protein disorders in the nematode Caenorhabditis elegans.

Methodology

1. Design of Fluorescent Protein Reporters
   We will design dual-colour fluorescent protein reporters to monitor the misfolding and aggregation of disease-linked myosin variants, our model proteins.

2. Creation of the ‘MyoState’ Library
   The ‘MyoState’ library, comprising hundreds of mutants with a broad range of folding defects, will allow us to study the pathological impact of individual myopathy mutations and investigate cellular rescue mechanisms.

Animal Model and Genome-Wide Screens

In C. elegans, myosin disease mutations cause characteristic defects in motility and muscle structure, which can be rescued by dietary restriction. This animal model thus provides us with a unique opportunity to perform genome-wide screens that will define the PQC network in muscle cells.

Structural and Functional Analyses

By performing structural and functional analyses of key factors in this network, we aim to reveal the fundamental mechanisms of myosin quality control and its regulation.

Ultimate Goal

Our ultimate goal is to understand how the misfolding of functional proteins, whether due to mutation or age, impairs cell function.