SubsidieMeestersOncolipidomics: Why is lipidomic dysregulation pattern in blood similar for various cancers?
The project aims to develop advanced lipidomic techniques to create a Cancer Lipidome Atlas, enhancing early cancer detection and treatment through comprehensive lipid profiling and data integration.
Projectdetails
Introduction
Lipids are involved in numerous pathways of human metabolism that are related to pathological states. Alterations of lipid concentrations in the blood of cancer patients have been reported, but the biological origin is still unknown.
Importance of Lipid Dysregulation
Deciphering the mechanisms of the lipid dysregulation could dramatically change oncology because it can open new avenues for cancer detection with subsequent effective treatment and drug development targeting dysregulated pathways. Early cancer diagnosis is one of the main unmet needs in medicine, which can improve the unfavorable prognosis of patients.
Limitations of Current Lipidomics
The potential of lipidomics has not been fully explored yet because analytical workflows have limitations in terms of accurate molar quantitation and insufficient coverage of the lipidome. Biologists predict up to 100,000 lipid species in nature, but current methods typically report less than 1% of this number.
Proposed Methodology
Here, we will develop novel approaches for quantitation of more than 2,000 lipids from over 80 classes using:
- 13C stable isotope labeled internal standards
- Ultrahigh-resolution methods in liquid or supercritical fluid chromatography
- Mass spectrometry
- Ion mobility
The comprehensive characterization of the lipidome will allow us to construct a Cancer Lipidome Atlas (WP1).
Software Development
We will develop new Bayesian software for automated data processing and statistical evaluation applicable to the main lipidomic and metabolomic workflows (WP2).
Correlation with Other Omics
We will correlate lipidomics data with metabolomics, proteomics, and transcriptomics data to unravel why lipidomic dysregulation in blood has a similar pattern for various cancers (WP3).
Comparative Analysis
This strategy will be applied for the comparison of ten types of cancer with control samples in:
- Cell lines
- Animal models (mice and pigs)
- Human samples (tissues and plasma)
- Extracellular vesicles
Hypothesis
Our initial hypothesis is that the lower activity of CERS2 triggered by cancer cells can downregulate very long fatty acyl ceramides and other sphingolipids.
Financiële details & Tijdlijn
Financiële details
| Subsidiebedrag | € 3.499.413 |
| Totale projectbegroting | € 3.499.413 |
Tijdlijn
| Startdatum | 1-8-2023 |
| Einddatum | 31-7-2028 |
| Subsidiejaar | 2023 |
Partners & Locaties
Projectpartners
- UNIVERZITA PARDUBICEpenvoerder
Land(en)
Vergelijkbare projecten binnen European Research Council
| Project | Regeling | Bedrag | Jaar | Actie |
|---|---|---|---|---|
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Elucidating the phenotypic convergence of proliferation reduction under growth-induced pressureThe UnderPressure project aims to investigate how mechanical constraints from 3D crowding affect cell proliferation and signaling in various organisms, with potential applications in reducing cancer chemoresistance. | ERC STG | € 1.498.280 | 2022 | Details |
Uncovering the mechanisms of action of an antiviral bacteriumThis project aims to uncover the mechanisms behind Wolbachia's antiviral protection in insects and develop tools for studying symbiont gene function. | ERC STG | € 1.500.000 | 2023 | Details |
The Ethics of Loneliness and SociabilityThis project aims to develop a normative theory of loneliness by analyzing ethical responsibilities of individuals and societies to prevent and alleviate loneliness, establishing a new philosophical sub-field. | ERC STG | € 1.025.860 | 2023 | Details |
MANUNKIND: Determinants and Dynamics of Collaborative Exploitation
This project aims to develop a game theoretic framework to analyze the psychological and strategic dynamics of collaborative exploitation, informing policies to combat modern slavery.
Elucidating the phenotypic convergence of proliferation reduction under growth-induced pressure
The UnderPressure project aims to investigate how mechanical constraints from 3D crowding affect cell proliferation and signaling in various organisms, with potential applications in reducing cancer chemoresistance.
Uncovering the mechanisms of action of an antiviral bacterium
This project aims to uncover the mechanisms behind Wolbachia's antiviral protection in insects and develop tools for studying symbiont gene function.
The Ethics of Loneliness and Sociability
This project aims to develop a normative theory of loneliness by analyzing ethical responsibilities of individuals and societies to prevent and alleviate loneliness, establishing a new philosophical sub-field.
Vergelijkbare projecten uit andere regelingen
| Project | Regeling | Bedrag | Jaar | Actie |
|---|---|---|---|---|
Proteomic Analysis of Cell communication in TumorsThis project aims to analyze cancer proteome dynamics at single-cell resolution to understand tumor heterogeneity and improve personalized treatment for resistant metastatic cells. | ERC COG | € 2.000.000 | 2022 | Details |
Comprehensive Platform for the Functional Characterization of Cancer Epigenetics and DiagnosisEpiCancer aims to develop single-cell epigenetic analysis tools to understand cancer heterogeneity and improve diagnostics through blood tests, enhancing early detection and monitoring of tumors. | ERC STG | € 1.500.000 | 2024 | Details |
An Atlas of Organisation of Lipids in Extracellular Vesicles To Navigate Their Roles in Cancer MetastasisThis project aims to create an atlas of asymmetric lipid compositions in cancer-derived extracellular vesicles to understand their role in metastasis using advanced imaging techniques. | ERC STG | € 2.499.818 | 2024 | Details |
Targeting Palmitic Acid Signaling Machinery to Inhibit Metastatic CancerPalmitoMET seeks to discover inhibitors of specific protein lipidation to combat cancer metastasis, aiming to develop first-in-class drugs for preclinical and clinical applications. | ERC POC | € 150.000 | 2023 | Details |
Proteomic Analysis of Cell communication in Tumors
This project aims to analyze cancer proteome dynamics at single-cell resolution to understand tumor heterogeneity and improve personalized treatment for resistant metastatic cells.
Comprehensive Platform for the Functional Characterization of Cancer Epigenetics and Diagnosis
EpiCancer aims to develop single-cell epigenetic analysis tools to understand cancer heterogeneity and improve diagnostics through blood tests, enhancing early detection and monitoring of tumors.
An Atlas of Organisation of Lipids in Extracellular Vesicles To Navigate Their Roles in Cancer Metastasis
This project aims to create an atlas of asymmetric lipid compositions in cancer-derived extracellular vesicles to understand their role in metastasis using advanced imaging techniques.
Targeting Palmitic Acid Signaling Machinery to Inhibit Metastatic Cancer
PalmitoMET seeks to discover inhibitors of specific protein lipidation to combat cancer metastasis, aiming to develop first-in-class drugs for preclinical and clinical applications.