Leveraging CRISPR-Cas for fast and accurate point-of-care diagnostics

Introduction

In the healthcare sector, rapid and precise point-of-care diagnostics is indispensable for enhancing patient outcomes, particularly in critical scenarios such as sepsis and organ transplantations.

Current Diagnostic Technologies

Present In Vitro Diagnostic technologies often strike a compromise between accuracy, speed, portability, and cost-effectiveness.

• Immunoassays offer rapidity but sacrifice accuracy.
• Molecular diagnostics like PCR deliver accuracy at the expense of time and ease of use.
• Isothermal molecular tests, on the other hand, are swift but lack specificity.

Hence, a pressing need persists for point-of-care diagnostic tools that embody user-friendliness, cost-efficiency, high accuracy, and adaptability to diverse applications.

Scope Biosciences Solution

At Scope Biosciences, a spin-off from Wageningen University, we have developed and patented a CRISPR-Cas diagnostic technology (scopeDx) that harnesses the precision of PCR and the speed of isothermal amplification, delivering rapid results within approximately 30 minutes at the point of care. Furthermore, the scopeDx platform can be rapidly adapted to new applications.

Objectives

Our objective is to complete the pre-clinical validation of our solution to offer:

1. User-friendliness
2. Robustness
3. Portability
4. Cost-effectiveness

This will provide swift results directly at the point of care.

Versatility of the Platform

Besides, we aim to prove the versatility of our platform, which can be extended to the detection of various pathogenic microbes (viruses, bacteria, fungi) and genomic applications (stem-cell & tissue transplantations).

Conclusion

In essence, scopeDx constitutes a modular platform technology with the potential to revolutionize the point-of-care diagnostics market comprehensively.